Identification and elucidation of cross talk between SLAM Family Member 7 (SLAMF7) and Toll-like receptor (TLR) pathways in monocytes and macrophages

To further elucidate the expression, regulation and function of Signaling Lymphocytic Activation Molecule Family (SLAMF) protein members in human monocytes and macrophages. Un-differentiated monocytic THP-1 cell (u-THP-1) and differentiated THP-1 macrophage (d-THP-1) were used as culture models in the study. Responses of cells to the differentiation agents phorbol ester (25 ng/ml) and TLR (Toll-like receptor) ligands were assessed. RT-PCR and Western blot analysis were used to determine mRNA and protein level. Pro-inflammatory cytokine mRNA expression levels and phagocytosis were used as functional markers. Data analyzed using t-test, one-way or two-way ANOVA followed by post hoc test. SLAMFs were differentially expressed in THP-1 cells. Differentiation of u-THP-1 to d-THP-1 led to significantly higher SLAMF7 mRNA and protein levels than other SLAMF. In addition, TLR stimuli increased SLAMF7 mRNA expression but not protein expression. Importantly, SLAMF7 agonist antibody and TLR ligands synergistically increased the mRNA expression levels of IL-1β, IL-6 and TNF-α, but had no effect on phagocytosis. SLAMF7 knocked-down in d-THP-1 significantly lowered TLR-induced mRNA expressions of pro-inflammatory markers. SLAM family proteins are differentially regulated by differentiation and TLRs. SLAMF7 enhanced TLR-mediated induction of pro-inflammatory cytokines in monocytes and macrophages but not phagocytosis.


Identification and elucidation of cross talk between SLAM Family Member 7 (SLAMF7) and Toll-like receptor (TLR) pathways in monocytes and macrophages
Uyory Choe 1 , Quynhchi Pham 2 , Young S. Kim 3

To further elucidate the expression, regulation and function of Signaling Lymphocytic Activation Molecule Family (SLAMF) protein members in human monocytes and macrophages. Un-differentiated monocytic THP-1 cell (u-THP-1) and differentiated THP-1 macrophage (d-THP-1) were used as culture models in the study. Responses of cells to the differentiation agents phorbol ester (25 ng/ml) and TLR (Toll-like receptor) ligands were assessed. RT-PCR and
Western blot analysis were used to determine mRNA and protein level. Pro-inflammatory cytokine mRNA expression levels and phagocytosis were used as functional markers. Data analyzed using t-test, one-way or two-way ANOVA followed by post hoc test. SLAMFs were differentially expressed in THP-1 cells. Differentiation of u-THP-1 to d-THP-1 led to significantly higher SLAMF7 mRNA and protein levels than other SLAMF. In addition, TLR stimuli increased SLAMF7 mRNA expression but not protein expression. Importantly, SLAMF7 agonist antibody and TLR ligands synergistically increased the mRNA expression levels of IL-1β, IL-6 and TNF-α, but had no effect on phagocytosis. SLAMF7 knocked-down in d-THP-1 significantly lowered TLR-induced mRNA expressions of pro-inflammatory markers. SLAM family proteins are differentially regulated by differentiation and TLRs. SLAMF7 enhanced TLR-mediated induction of proinflammatory cytokines in monocytes and macrophages but not phagocytosis.
Abbreviations d-THP-1 Differentiated THP-1 macrophage u-THP-1 Undifferentiated THP-1 monocyte LPS Lipopolysaccharide IL-1β Interleukin 1 beta IL- 6 Interleukin 6 CCL2 Chemokine ligand 2 TNF-α Tumor necrosis factor alpha COX-2 Cyclooxygenase 2 PGE 2 Prostaglandin E2 IFN-γ Interferon gamma Inflammatory pathways play a vital role in response to many insults such as injury, infection, trauma 1 and are regulated by immune cells including monocytes and macrophages. During inflammation, monocytes serve as immune effector cells and are equipped with chemokine receptors and adhesion receptors for the role 2 . Monocytes produce pro-inflammatory cytokines such as IL-1β, IL-6, CCL2 and TNF-α, and remove cell debris and pathogens through phagocytosis. Additionally, monocytes are recruited to the site of inflammation in a tissue where they can be differentiated into macrophages 3 . This differentiation process is important since production of pro-inflammatory cytokines such as IL-1β, IL-6, CCL2 and TNF-α by macrophages is critical for an acute inflammatory response 4 . Macrophages also engulf debris, foreign substances, microbes, and cancer cells through phagocytosis. In addition, macrophages play a critical role in innate immunity and help initiate adaptive immunity by recruiting other immune cells such as the lymphocytes 5 . The SLAM family proteins are a group of type I transmembrane receptors present in immune cells such as monocytes, macrophages, natural killer (NK) cells, CD8+ T lymphocytes, B lymphocytes and mature dendritic cells 6,7 . The SLAMF receptors currently have 9 members with different names in the literature, which include SLAMF1 (SLAM or CD150), SLAMF2 (CD48), SLAMF3 (Ly-9 or CD229), SLAMF4 (2B4 or CD244), SLAMF5 (CD84), SLAMF6 (Ly108 in mice, NTB-A or SF2000 in humans), SLAMF7 (CRACC, CD319 or CS1), SLAMF8 (CD353 or BLAME) and SLAMF9 (SF2001 or CD84H) [8][9][10][11] . The SLAMF receptors are important immunomodulatory receptors and have various functions including T cell activation [12][13][14] , Th2 cytokine production [13][14][15][16][17][18][19][20][21][22][23] , NK-and CD8+ T cells mediated cytotoxicity 24,25 . In general, SLAMF receptors are self-ligand i.e. each SLAM protein recognizes itself to trigger down-stream signaling pathways 26 . This interaction allows different immune cell types to interact with each other through SLAMF receptors 26 . One exception is SLAMF4, which recognizes SLAMF2 and vice versa 26 . The SLAMF receptors are usually composed of two immunoglobulin (Ig) like domains, one variable (V) like domain and one constant 2 (C2) like domain 27 ; except SLAMF3 which has four Ig-like domains with two repeated patterns of V-like and C2-like domains 27 . The signal transduction of SLAMF receptors is mediated through signaling lymphocyte activation molecule-associated protein (SAP) adaptors. The SAP adaptors are comprised of SH2 Domain Containing 1A (SAP), SH2 Domain Containing 1B (EAT-2) and EAT-2-related transducer (ERT). SAP adaptors are small proteins that consist of a single Src homology 2 (SH2) domain. Through the SH2 domain, the SAP adaptors interact with immunoreceptor tyrosine-based motifs (ITSMs) in the cytoplasmic domain of SLAMF receptors. Among SAP adaptors, EAT-2 appears to be receptor-specific. For example, in human NK-cells, SLAMF7 recruits exclusively EAT-2 but not SAP 27 . SLAMF receptors can induce signals through interaction with SAP adaptors to various downstream effectors such as nuclear factor of activated T-cells (NFAT), nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) and vav guanine nucleotide exchange factor 1 (Vav1) to exert their biological activities 28 . However, some exceptions exist. For example, SLAMF2 is an atypical SLAMF member that has no cytoplasmic region and thus lacks type I transmembrane glycoproteins but has a glycosylphosphatidylinositol membrane anchor 27 . SLAMF8 and SLAMF9 have a short cytoplasmic tail that lacks the ITSM, similar to SLAMF2, and have no known ligands 27 . Although many studies have been reported for SLAMF receptors in T-and NK-cells 9,26,29 , the literature on the function of SLAMF receptors in monocytes and macrophages remain scarce.
Existing literature suggests that the SLAMF proteins and TLRs appear to interact with each other 30,31 . The TLRs are pattern recognition receptors present in immune cells and play an important role as a defense mechanism against bacterial and viral infection 32 . For examples, the gram-negative bacterial cell wall component, LPS, can bind to TLR4. Flagellin, the primary protein component of the flagellar filament in bacteria, can bind to TLR5. Also, bacterial lipoprotein can bind to TLR2/6 and TLR1/2 and synthetic lipopeptides such as Pam2CSK4 and Pam3CSK4 are often used to investigate this pathway 33,34 . When ligands bind to TLRs, signal transduction through transcriptional factors such as NF-κB and AP-1 are activated. Activation of NF-κB and AP-1 induce pro-inflammatory cytokines (TNF-α, IL-1β and IL-6) and chemokines (CCL2 and CXCL8) leading to inflammatory responses which include endothelial cell activation, neutrophil granulocyte activation, fever induction, acute phase protein synthesis, and B lymphocytes proliferation 35 . Farina and others reported that SLAMF1 can be induced by engagement of TLR2, 4 and 5 30 . Sintes et al. 31 reported that SLAMF5 modulate signaling pathways downstream of TLR4. LPS-stimulated secretion of two pro-inflammatory cytokines (IL-6 and TNF-α) were significantly reduced when RAW-264.7 macrophage cells were transfected with SLAMF5 siRNA 31 . Hence, cross talk between the SLAMF and TLR pathways exist but the precise relationship between specific SLAMF receptors and TLRs remain unclear.
In addition to TLR pathways, the differentiation of monocytes to macrophages may play a critical role in SLAMF protein regulation. Previously, our lab found that SLAMF5 (CD84) mRNA as well as protein levels were up-regulated when phorbol 12-myristate 13-acetate (PMA) was used to induce differentiation of u-THP-1 cells (monocytes) to d-THP-1 cells (macrophages) 36 . Therefore, it is possible that other SLAMFs may also be subjected to similar regulation as SLAMF5, but this conjecture warrants further elucidation.
Recently, SLAMF7 also received much attention due to their high expression in multiple myeloma and therefore could be explored as a potential therapeutic target 37 . This led to the development of Elotuzumab, a novel humanized immunoglobulin G1 (IgG1) monoclonal antibody which targets SLAMF7 on myeloma cancer cell. Elotuzumab has recently been approved for the treatment of relapsed or refractory multiple myeloma in combination with lenalidomide and dexamethasone 38 . The mechanisms appeared to involve activation of NK cell as well as activation of macrophage phagocytosis to eradicate tumor cells 39 . In addition, SLAMF7-mediated macrophage phagocytosis was also reported to be involved in anti-CD47-induced phagocytosis of hematopoietic tumor cells 40 . This effect appeared to be SAP-independent 40 . Finally, SLAMF7 is shown to play a significant role in macrophage-related inflammatory diseases such as rheumatoid arthritis, Crohn's disease, and COVID-19 infection 41 . However, the role of SLAMF7 and other SLAMs in normal inflammatory processes such as phagocytosis in monocytes and macrophages are less well known and warrant elucidation.
Questions regarding the role of SLAMF proteins and their mechanisms on regulation especially in monocytes and macrophages remain unclear. In this study, we focus on examining the role of SLAMF members in common inflammatory response, such as the interaction with bacterial products to determine 1) if SLAMF proteins are regulated during the differentiation process from monocytes to macrophages, 2) if SLAMF proteins are regulated by the TLRs in monocytes and macrophages, and 3) if SLAMF proteins modulate TLR stimuli-induced production of cytokines and phagocytosis. We focused on SLAMF 1, 3, 4, 5, 6, 7 excluding SLAMF2, SLAMF8 and SLAMF9 due to their atypical composition i.e. lacking ITSM, from other SLAMF members. Our results indicated that SLAMF proteins were differentially regulated by cellular differentiation and TLRs, and SLAMF7 www.nature.com/scientificreports/ was involved in enhancing TLR-mediated induction of pro-inflammatory cytokines but not phagocytosis in monocytes and macrophages.

Materials and methods
Materials and reagents. Flagellin  Briefly, the siRNA/HiPerFect complex was made and added dropwise to PMA (25 ng/ml) activated THP-1. The whole cell/complexes solution was incubated for 6 h, then additional fresh cell culture media in the presence of 10% FBS and 1% Pen/Strep was added to each well. Plates were then incubated for 48 h to facilitate transfection followed by with/without LPS incubation for an additional 4 h. After LPS treatment, cells were harvested for RNA isolation. Relative mRNA levels for SLAMF7, IL-1β, IL-6, TNF-α, and CCL2 were determined using RT-PCR as described below.
Total RNA isolation, cDNA synthesis, and Real-time PCR. Total RNA was isolated by using the TRIzol reagent as previously described 36 . Real-time PCR was used for quantifying changes in relative mRNA levels. 1 µg of total RNA was used for cDNA synthesis using the AffinityScript Multi Temperature cDNA Synthesis kit according to the manufacturer's protocol. Real-time PCR was performed using the TaqMan Fast Universal PCR Master Mix according to the previously published protocol on ViiA7 real-time PCR system following the manufacturer's protocol 36 . For the determination of relative mRNA levels, TATA binding protein (Tbp) was used as housekeeping gene for normalization. Relative expression value was generated using Ct method as described by the manufacturer's protocol. The following TaqMan Gene Expression Assays (Thermo Fisher Scientific, Waltham, MA) PCR primer/probe sets were used: SLAMF1(Hs00234149_m1), SLAMF3 (Hs03004331_m1), SLAMF4 (Hs00175568_m1), SLAMF5 (Hs01547121_m1), SLAMF6 (Hs00372941_m1), SLAMF7 (Hs00221793_m1), IL-1b (Hs01555410_m1), IL-6 (Hs00985689_m1), CCL2 (Hs00234140_m1), and TNF-aα (Hs00174128_m1).
Western blot analysis. Western analysis of protein expression was conducted as described previously 36 .
Briefly, after treatments cultured u-THP-1 cells were harvested by centrifugation at 400 RCF for 5 min, cell pellets were washed twice with cold PBS and centrifuge at 400 RCF for 5 min, supernatant removed. Cell pellets were immediately mixed with 100 μL of radio immunoprecipitation assay (RIPA) buffer containing EDTA, protease and phosphatase inhibitors.

Statistical analysis.
GraphPad Prism (Prism 9, GraphPad Software Inc., La Jolla, CA, USA) was used for statistical analysis 42 . Each experiment was performed in triplicate. Data were expressed as mean ± SD. For two groups comparison, t-test was used. For multiple groups either one-way or two-way ANOVA followed by posthoc test (Tukey test) was used depending on experimental design. In all cases, p ≤ 0.05 was considered significant.

Expression of SLAMF mRNAs and proteins in the human THP-1 monocytes, THP-1 macrophages and Jurkat cells.
We first examined the relative expression of SLAMF mRNAs in human THP-1 cells. As shown in Fig. 1A, SLAMF4 and SLAMF5 are the most highly expressed mRNA in SLAMF proteins in u-THP-1 while other SLAMF members' expressions were minimal. Interestingly, in d-THP-1, SLAMF5, SLAMF7 were highly expressed, followed by SLAMF4 as the third most highly expressed SLAMF member (Fig. 1A). To further elucidate specificity of SLAMF protein expression in different immune cells, Jurkat cell, a lymphocyte cell model, was also queried for SLAMF as comparison to macrophage/monocyte. In contrast to THP-1 cells, in Jurkat cells (Fig. 1B), SLAMF5 appeared to be the most highly expressed SLAMF protein. In contrast to THP-1 macrophage, SLAMF7 mRNA expression was minimal in Jurkat cells. Also, a significant up-regulation of SLAMF5, SLAMF7 but not SLAMF4 mRNA expression levels were detected during the PMA-induced differentiation of u-THP-1 to d-THP-1. The up-regulated mRNA expression levels of SLAMF5 and 7 were confirmed at the protein level (Fig. 2). In contrast to SLAMF5, two immune-detectable SLAMF7 bands were observed. A 37 kDa and a 50 kDa, representing the original molecular weight of SLAMF7 and its presumable glycosylated form 38 , respectively. PMA induction of THP-1 cells led to time-dependent increase in both immune-detectable SLAMF5 and SLAMF7 proteins (Fig. 2).

Effect of TLRs activation on SLAMF mRNA levels in monocytes and macrophages.
We next determined the effects of TLR activation on SLAMFs given the role of TLR in host's immune responses. SLAMF protein mRNA expression levels were evaluated when u-THP-1 and d-THP-1 cells were activated with specific TLR ligands. As shown in Fig. 3, only SLAMF7 was significantly induced by all four TLR stimuli including LPS, Flagellin, Pam2CSK4 and Pam3CSK4. Compared to SLAMF7 mRNA expression levels, other SLAMF protein expression levels were minimal. Since we previously published work on expression, regulation and immune-related roles of SLAMF5 36 and given the uniqueness of SLAMF7 expression, regulation patterns, we focused on SLAMF7 for follow-up analysis.

Characterization of LPS responses of SLAMF7 in monocyte (u-THP-1) and macrophage (d-THP-1). CD14 dependency of LPS-induced increase in SLAMF7 mRNA levels.
Because TLR4 can be activated through CD14-dependent or independent pathway, we further characterized the CD14 dependency of the TLR4 ligand LPS induction of SLAMF7 mRNA levels. The pathway of LPS-induced SLAMF7 mRNA expressions in u-THP-1 and d-THP-1 was evaluated using CD14 blocking antibody. Blocking CD14 in both u-THP-1 and d-THP-1 significantly reduced LPS-induced SLAMF7 mRNA expression levels (Fig. 4A).
Time-dependent effects of LPS on SLAMF7 mRNA levels. The mRNA expression levels of SLAMF7 in u-THP-1 cells stimulated with LPS peaked at 4 h and then decreased. In d-THP-1 cells, mRNA expression levels of SLAMF7 stimulated with LPS peaked at 6 h and then decreased (Fig. 4B).
Concentration dependent effect of LPS on SLAMF7 mRNA levels. LPS induced a concentration dependent increase in SLAMF7 mRNA expression levels in both u-THP-1 and d-THP-1 cells (Fig. 4C). Induction of SLAMF7 mRNA levels in both u-THP-1 and d-THP1 cells was significantly induced by LPS at 1 ng/ml. www.nature.com/scientificreports/ LPS stimulation affect SLAMF7 at protein level. We also seek to confirm LPS stimulation of SLAMF7 at the protein level. TLRs activation significantly induced SLAMF7 protein expression (Fig. 5).

Effects of SLAMF7 siRNA knocked-down in d-THP-1 on LPS-induced cytokines. The unique
expression and regulation of SLAMF7 in macrophage led us to evaluate the functional roles of SLAMF7 in monocyte/macrophage. Using siRNA knockdown experiment, SLAMF7 knock-down in d-THP-d significantly reduced SLAMF7 mRNA expression levels with and without LPS stimulation (Fig. 6A). In the presence of LPS, SLAMF7 knock-down in d-THP-1 significantly attenuated LPS-induced mRNA expression levels of pro-inflammatory cytokines including IL-1β, IL-6, CCL2 and TNF-α compared to the non-target control (NC) (Fig. 6B-E).

Identifying SLAMF7 activating antibody and its effect on TLR stimuli-induction of cytokines.
Based on reported Elotuzumab activating property 38,39 , we seek to identify commercially available antibody that can activate SLAMF7. We found that addition of the SLAMF7 antibody (Cat#:NBP2-45868), but not IgG 1 control antibody (Cat#:sc-52003), enhanced up-regulation of pro-inflammatory cytokines including IL-1β, IL-6 and TNF-α in u-THP-1 cells by TLR ligands LPS, Flagellin, Pam2CSK4 or Pam3CSK4, (Fig. 7, S1 and S3). Similarly, when d-THP-1 was activated with LPS, Flagellin or Pam3CSK4 in presence of SLAMF7 agonist antibody, enhanced up-regulation of pro-inflammatory cytokines including IL-1β, IL-6 and TNF-α was also observed. In contrast, activation with Pam2CSK4 in the presence of SLAMF7 agonist antibody slightly lowered IL-6 and TNF-α mRNA expression levels compared to its IgG 1 control (Fig. S1-S3).
SLAMF7's effect on macrophages (d-THP-1) phagocytosis activity. In addition to cytokine production, we also asked if SLAMF7 played a role in phagocytotic activity of macrophage. Using the SLAMF7 ago- www.nature.com/scientificreports/ nist antibody described above, that enhanced cytokine production, we did not observe an effect of the antibody on d-THP-1 phagocytosis activity as compared to IgG 1 or a positive control (Fig. 8).

Discussion
In the present study, several important pieces of information related to SLAMF proteins were identified which included: 1) Identification of crosstalk between SLAMF7 and several selected TLRs in the monocyte/macrophage. 2) SLAMF7 to be the major SLAMF protein regulated in monocyte/macrophage in term of fold changes. 2) Different types of immune cells express different sets of SLAMF protein may indicate functional selectivity. 3) Differentiation and TLR stimuli regulated specific SLAMF members in monocyte/macrophage. 4) Functionally, SLAMF7 activation enhanced selected TLR-induced cytokine responses but not phagocytosis. The SLAMF receptors are important immunomodulatory receptors in the immune cells. These receptors are known to have a wide spectrum of roles including regulation of cytotoxicity, humoral immunity, autoimmunity, cell survival, lymphocyte development, cell adhesion, invasiveness, and production of cytokines [6][7][8][9][10][11][12][13][14][15][16][17][18][19][20][21][22][23][24][25]43,44 . Moreover, due to having multiple members in the SLAMF receptors, their expression patterns in immune cells such as monocytes and macrophages remained unclear but may be important contributors to their function. Our results indicated that monocyte SLAMF4 and 5 appeared to be the dominant SLAMF in monocytes; which was consistent with previously published reports 24,25 . These results suggested that SLAMF4 and 5 may play a relatively more central role than other SLAMF in mediating monocyte's function.
Differentiation of monocyte to macrophage significantly altered the expression profile of SLAMF protein; most notably, an induction of SLAMF7 expression. Comparing human T lymphocyte Jurkat cells to THP-1 monocyte/macrophage (Fig. 1B), SLAMF7 showed unique expression in the macrophage, suggesting a specific functional role for this protein in immune responses of macrophages. The biological efficacies specific to SLAMF7 were confirmed from experiments using siRNA against SLAMF7. Transient transfection with SLAMF7 siRNA to knock-down SLAMF7 expression in d-THP-1 significantly reduced LPS activated mRNA expression levels of pro-inflammatory cytokines including IL-1β, IL-6, CCL2 and TNF-α (Fig. 6A-E). These data support SLAMF7 play an enhancing role in mediating the effects of inflammatory stimuli in macrophage. Unfortunately, the efficiency of siRNA for u-THP-1 was too low (data not shown), therefore, experiments in u-THP-1 were not able to be conducted. This data is consistent with reported literature that u-THP-1 cells are notoriously difficult to transfect 45 . Importantly, we identified a SLAMF7 antibody that function as an agonist antibody.
The SLAMF7 antibody (Cat#NBP2-45868), enhanced up-regulation of pro-inflammatory cytokines including IL-1β, IL-6 and TNF-α mRNA by TLR ligands LPS (TLR4), Flagellin (TLR5) and Pam3CSK4 (TLR1/2) (Fig. 7,   Figure 2. Western blot analysis of SLAMF5 and SLAMF7 protein expression in monocytes (u-THP-1) and macrophages (d-THP-1). Cells were cultured and SLAMF protein levels were determined by Western blot analysis. The proteins were detected and quantified using the LICOR ODYSSEY® CLx (LiCOR, Lincoln, NE, USA) Infrared Imager according to manufacturer's procedure 37 . Results are expressed as mean ± SD (n = 3). Bars with asterisk indicate significant difference to that of u-THP-1 monocytes www.nature.com/scientificreports/ S1-S3) in both d-and u-THP-1 cells. These data are consistent with a recent report by Simmons et al. 41 and provide independent verification of an enhanced immune response by SLAMF7 engagement. One exception from our study was observed in Pam2CSK4 (TLR2/6 ligand)-activated d-THP-1 ( Fig. S1-S3). In this case, the SLAMF7 agonist antibody and Pam2CSK4 did not synergistically induce IL-6, CCL2 and TNF-α. These results suggested that SLAMF7 may only interact with selective TLR to enhance the immune responses elicit by those TLRs, but more studies are needed to further elucidate the specific differences. In addition, the chemokine CCL2 showed a slightly different mRNA expression levels compared to the other cytokines (Fig. S2) suggesting that selective modulation of specific cytokines/chemokines pathways by SLAMF7 may also exist. Overall, our results from siRNA and agonist antibody supported involvement of SLAMF7 in the regulation of selected TLR-induced proinflammatory cytokines/chemokines in u-THP-1 and d-THP-1. This cross talk between SLAMF7 and selected TLR allow for the monocyte/macrophage to mount a concerted robust immune response during infection. Of note, the agonist antibody enhanced the selected TLR-induction of cytokines (Fig. 7) only in the presence of TLR ligands. Hence, SLAMF7 engagement itself is not capable of directly induce cytokines but rather SLAMF7 appeared to play a supporting role in the process. The interaction between TLR and SLAMF7 appeared to be through CD14-related pathway as knock-down of CD14 inhibited the induction by LPS (Fig. 4A). We also evaluated SLAMF7 biological efficacy in phagocytosis, as phagocytosis is a major mechanism by which monocyte/macrophage remove pathogens and cell debris 46 . However, we did not observed effects of agonist antibody on phagocytosis. These results and the cytokine analysis supported the cross talk between SLAMF7 and TLR pathways that regulates cytokine production but not phagocytosis.
Several other SLAMF were also upregulated during differentiation (Fig. 1A). Therefore, differentiation is apparently the main driver for regulating the SLAMF pathway. Although we have not tested all up-regulated SLAMF pathways, we suspect the SLAMF pathway provide overall promotional effects on host's immune response through interaction with TLR-associated pathways. SLAMF1, 4 appeared not to be regulated by differentiation or TLR stimuli, therefore may not contribute to enhanced immune responses, such as cytokine production, in the macrophage. However, additional studies are needed to further elucidate the specific role for other SLAMF not examined in this study in monocyte/macrophage.

Conclusion
Our study indicated that SLAMF receptors are differentially expressed in immune cells. We also provided data to support differentiation from monocytes to macrophages lead to up-regulation of SLAMF7 that promote induction of cytokines by selected TLR ligands in monocytes and macrophages. Overall, our results indicate crosstalk and a coordinated response of selected TLR and SLAMF7-mediated pathways against immune stimuli in monocyte/macrophage exist.

Data availability
The datasets used and/or analyzed in the current study are available from the corresponding author on reasonable request.
Received: 3 March 2023; Accepted: 14 June 2023 Figure 8. Effects of SLAMF7 antibody on phagocytosis activity of macrophage (d-THP-1). U-THP-1 cells were differentiated to d-THP-1 cells and phagocytosis activity was determined in the presence or absence of control antibody or SLAMF7 antibody. B: Blank (without E. coli particle) or negative control, and Ctrl: E. coli particles were used as a phagocytosis pathogen or positive control. +IgG: with isotype IgG control added, +Ab-SLAMF7: with antibody against SLAMF7 added. Results are expressed as mean ± SD (n = 3). Bars with different letters indicate significant difference at p ≤ 0.05.